Department of Biology
Phone: +91- (0)877 2500 908
Our laboratory investigates the molecular mechanisms that underpin the biological questions in the following major research areas by employing the integrative structural biology (cryo-electron microscopy, X-ray crystallography, bioinformatics and molecular dynamics) and chemical-biology approach.
Natural product biosynthesis (NPB): Elucidating the molecular basis of NPB towards harnessing novel antibiotics to combat the antimicrobial resistance. To overproduction of these novel antibiotics, it is essential to fine-tune the control by the transcription regulators over the biosynthesis. Hence, investigations toward the comprehensive understanding of both the NPB and the control over its onset will enable us to harbour novel antibiotics at large scale to meet the need fighting the escalating problem of antimicrobial resistance.
Plant immunity: Plants have an ability to counter the pathogen infection however, in most cases, their immune system is compromised and hence the infection. So, it is important to investigate this biological problem in order to potentiate the plant immune response towards minimizing the crop loss.
Bioinformatics and Machine learning: We also employ the power of computational biology and deep neuronal network models in machine learning to predict the interaction specificity between the biomolecules and also to predict the unknown DNA binding sequence for a transcription regulator. Both these models (DeepNAP and OpSeqFind) will be available as online tools for public use soon. Moreover, we have also developed a user-friendly web service (ASSAP 1.0) for the bioinformatics analysis, this online service integrates a wide range of best performing open-source tools on a single platform. It is anticipated that ASSAP 1.0 benefits both the bioscience community and educational purposes.
Education and career
- Ph.D. (2017): IIT Bombay and Monash University (joint PhD program).
- Post-doctoral researcher (2017 – 2020): Monash University, Australia.
- DST-INSPIRE Faculty (2021 – ): IISER Tirupati
- Shanshan Zhou†, Hussain Bhukya†, Nicolas Malet†, Peter Harison, Dean Rea, Matthew J. Belousoff, Hariprasad Venugopal, Paulina K. Sydor, Kathryn Styles, Lijiang Song, Max J. Cryle, Vilmos Fülöp, Gregory L. Challis and Christophe Corre "Molecular basis for control of antibiotic production by a bacterial hormone". Nature 2021, 590: 463−467 †equal contribution.
- Hussain Bhukya, Ruchika Bhujbalrao, Aruna Bitra and Ruchi Anand "Structural and functional basis of transcriptional regulation by TetR family protein CprB from S. coelicolor A3(2)". Nucleic Acids Res. 2014, 42(15):10122−10133.
- Hussain Bhukya, Asis K Jana, Neelanjana Sengupta and Ruchi Anand "Structural and dynamics studies of the TetR family protein, CprB from Streptomyces coelicolor in complex with its biological operator sequence". J. Struct. Biol. 2017, 198(2): 134−146.
- Anwesha Biswas, Ravi K. Swarnkar, Bhukya Hussain, Suraj K. Sahoo, P. I. Pradeepkumar, G. Naresh Patwari, and Ruchi Anand "Fluorescence quenching studies of γ-butyrolactone binding protein (CprB) from Streptomyces coelicolor A3(2)". J. Phys. Chem. B, 2014, 118(34): 10035−10042.
- Aruna Bitra, Bhukya Hussain, Ajay Singh Tanwar and Ruchi Anand "Identification of function and mechanistic insights of guanine deaminase from Nitrosomonas europaea: Role of the C-terminal loop in catalysis". Biochemistry, 2013, 52(20): 3512−3522.
- Hussain Bhukya and Ruchi Anand "TetR regulators ‒ A structural and functional perspective". J. Ind. Inst. Sci. 2017 97(2): 245−259.
- Siddhant Sharma – Integrated PhD student
- Arpita Das – PhD student
- Nitish Kumar – PhD student
- Aditya Bhaskarwar – PhD student
- Rachit Patil – Master’s Thesis student
- BIO322/622: Elements of Structural Biology
- BIO441/741: Biophysics
- BIO312/612: Biochemistry
- BIO111: Foundations of Biology I: Basic Principles
The lab has Open Positions for
- Ph.D. students (Apply through the IISER PhD admissions)
- Postdoc – please send your CV along with the research proposal that aligns with the research interest of the lab or it can be relatively new area where structural biology approach can be effectively used to address the biological question.